PyroBayes | Tool name | PyroBayes |
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| URL | http://bioinformatics.bc.edu/marthlab/PyroBayes |
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| Important features | 1. PyroBayes is a novel base caller for pyrosequences from the 454 sequencing machines.
2. Pyrobayes permits accurate SNP calling in re-sequencing applications, even in shallow read coverage, primarily because it produces more confident base calls than the native base calling program.
3. It produces more accurate higher base qualities and hence make more high quality base calls in 454 pyrosequences.
4. It produces base qualities up to 50. Pyrobayes can process a single sequencing run in under 2 minutes. |
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| Citations | Quinlan AR, Stewart DA, StrF6mberg MP, Marth GT. Pyrobayes: an improved base caller for SNP discovery in pyrosequences. Nat Methods. 2008 Feb;5(2):179-81. Epub 2008 Jan 13. PubMed PMID: 18193056. |
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| Year of publication | 2008 |
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| Rank by usage frequency | 100 |
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| Comments | |
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| Function | Base calling, SNP discovery |
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| Category | Free, Downloadable |
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| License | |
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| Status | |
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| Input file format | SFF |
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| Output file format | FASTA, FASTQ |
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| Operating system | Linux 32/62bit |
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| Operating language | |
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| Platform | Roche 454 |
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